Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_M1702:Design

Designed by: Qifei Li   Group: UtahState_BE5930_-_S11   (2013-04-17)


ScrR Promoter + RBS + ScrR + coding+Transcription terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1007
    Illegal AgeI site found at 3128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3213
    Illegal BsaI.rc site found at 3276


Design Notes

this is the composite part with promoter, RBS, ScrR, terminator and the coding area for Inulosucrase to digest fructose into inulin, which will not be absorbed by human body.

Source

The coding sequence is from Lactobacillus johnsonii NCC 533.

References

The Probiotic Lactobacillus johnsonii NCC 533 Produces High-Molecular-Mass Inulin from Sucrose by Using an Inulosucrase Enzyme, Munir A. Anwar, Slavko Kralj, Marc J. E. C. van der Maarel and Lubbert Dijkhuizen, Appl. Environ. Microbiol. June 2008 vol. 74 no. 11 3426-3433.